Barrera Laboratory

Membrane biophysics at the University of Tennessee Knoxville

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Protocols

  • Acrylamide Gels
  • Booking rooms in Mossman
  • CA protein expression protocol
  • CD protocol
  • Coomassie blue staining gel for polyacrylamide gels
  • Cuvette cleaning protocol
  • Exspresion y purificacion de la proteina CA
  • FACs protocol flow cytometry
  • Flow cytometer
  • FPLC
  • French press
  • Hemocytometer protocol
  • HPLC protocol for PHLIP and Alexa 488
  • Instructions for the Jasco J-815 CD
  • LB
  • Langmuir-Blodgett trough protocol
  • Lipid extrussion detailed Protocol
  • Micro-pH probe operation
  • OCD protocol
  • P-Tyr Protocol Dec’11
  • PHLIP protocol flow citometry
  • Phosphate Assay
  • pH measurement guide
  • Plate reader (BioTek) operation
  • Protocol Competent cell preperation
  • Protocol for translation of Alexa-555-pHLIP in to cells 1_17_2014
  • PTI fluorometer Protocol
  • Quick Change
  • Spectrophotometer protocol
  • Supported Lipid Bilayers for FRAP
  • TAE Buffer
  • Transformation protocol using Heat Shock
  • Miscelaneous Molecular Biology Protocols
  • Das lab confocal microscope
  • MALDI-TOF for Conjugations
  • MALDI sample preparation

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