Barrera lab undergraduate and graduate students are highlighted

2023

• Ancajas CF, Alam S, Alves DS, Zhou Y, Wadsworth NM, Cassilly CD, Ricks TJ, Carr AJ, Reynolds TB, Barrera FN, Best MD. “Cellular Labeling of Phosphatidylserine Using Clickable Serine Probes.” ACS Chem Biol. (2023).

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2022

• Russell CM, Rybak JA, Miao J, Peters BM, Barrera FN. “Candidalysin: connecting the pore forming mechanism of this virulence factor to its immunostimulatory properties.” J. Biol Chem. (2022).

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• Russell CM, Schaefer KG, Dixson A, Gray ALH, Pyron RJ, Alves DS, Moore N, Conley EA, Schuck RJ, White TA, Do TD, King GM, Barrera FN. “The Candida albicans virulence factor candidalysin polymerizes in solution to form membrane pores and damage epithelial cells.” eLife. (2022).

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• Lou J, Schuster JA, Barrera FN, Best MD. “ATP-Responsive Liposomes via Screening of Lipid Switches Designed to Undergo Conformational Changes upon Binding Phosphorylated Metabolites.” J. Am. Chem. Soc. (2022).

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• Barrera FN. “The advance that realized the potential of protein Fourier-transform infrared spectroscopy.” Arch. Biochem. Biophys. (2022).

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• Bogati B, Wadsworth N, Barrera FN, Fozo EM. “Improved growth of Escherichia coli in aminoglycoside antibiotics by the zor-orz toxin-antitoxin system.” J Bacteriol. (2022).

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• Schaefer KG,  Pittman AE,  Barrera FN, King GM. “Atomic force microscopy for quantitative understanding of peptide-induced lipid bilayer remodeling.” Methods. (2022).

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2021

• Aguilar M, Al Nahas K, Barrera FN, Bassereau P, Bastos M, Beales P, Bechinger B, Bonev B, Brand I, Chattopadhyay A, Clarke RJ, DeGrado W, Deplazes E, Garcia Saez AJ, Hoogenboom B, Lund R, Milán Rodríguez P, O’Shea P, Pabst G, Pal S, Roux A, Sanderson J, Semeraro EF, Sengupta D, Siegel DP, van ‘t Hag L, Vijayakumar A, Zoranić L. “Theoretical and experimental studies of complex peptide-membrane systems: general discussion.” Faraday Discuss. (2021).

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• Schaefer KG, Grau B, Moore N, Mingarro I, King GM, Barrera FN. “Controllable membrane remodeling by a modified fragment of the apoptotic protein Bax.” Faraday Discuss. (2021).

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• Aguilar M, Al Nahas K, Barrera FN, Bassereau P, Bastos M, Beales P, Bechinger B, Bonev B, Brand I, Chattopadhyay A, DeGrado W, Fuchs P, Garcia Saez AJ, Hoogenboom B, Kapoor S, Milán Rodríguez P, Molloy J, O’Shea P, Pabst G, Pal S, Rice A, Roux A, Sanderson J, Seddon J, Tamm LK, Vijayakumar A. “Behaviour and interactions of proteins and peptides with and within membranes; from simple models to cellular membranes: general discussion.” Faraday Discuss. (2021).

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• Aguilar MI, Al Nahas K, Barrera FN, Bassereau P, Bechinger B, Brand I, Chattopadhyay A, Clarke RJ, DeGrado WF, Deplazes E, Fletcher M, Fraternali F, Fuchs P, Garcia-Saez AJ, Gilbert R, Hoogenboom BW, Jarin Z, O’Shea P, Pabst G, Pal S, Sanderson JM, Seddon JM, Sengupta D, Siegel DP, Srivastava A, Tieleman DP, Tripathy M, Utterström J, Vácha R, Vanni S, Voth GA. “Theoretical and experimental comparisons of simple peptide-membrane systems; towards defining the reaction space: general discussion.” Faraday Discuss. (2021).

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• Liu J. , Willems HME , Sansevere EA, Allert S, Barker KS , Lowes DJ, Dixson AC, Xu Z, Miao J, DeJarnette C, Tournu H, Palmer GE, Richardson JP, Barrera FN, Hube B, Naglik JR, Peters BM. “A variant ECE1 allele contributes to reduced pathogenicity of Candida albicans during vulvovaginal candidiasis.” PLOS Pathogens. (2021).

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• Westerfield JM,  Sahoo AR, Alves DS, Grau B, Cameron A, Maxwell M, Schuster JA, Souza PCT, Mingarro I, Buck M, Barrera FN. “Conformational Clamping by a Membrane Ligand Activates the EphA2 Receptor.” JMB (2021).

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• Ward AE,  Ye Y, Schuster JA,  Wei S, Barrera FN. “Single-molecule fluorescence vistas of how lipids regulate membrane proteins.” Biochem Soc Trans (2021).

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• Ashkar R,  Doktorova M, Heberle FA,  Scott HL, Barrera FN, Katsaras J, Khelashvili G, Brown MF. “Reply to Nagle et al.: The universal stiffening effects of cholesterol on lipid membranes.” PNAS (2021).

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• Stefanski KM,  Russell CM, Westerfield JM,  Lamichhane R, Barrera FN. “PIP₂ promotes conformation-specific dimerization of the EphA2 membrane region.” J Biol. Chem. (2021).

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2020

• Katherine G. Schaefer,  Brayan Grau,  Nicolas Moore,  Ismael Mingarro, Gavin King  and  Francisco Barrera. “Controllable membrane remodeling by a modified fragment of the apoptotic protein Bax.” Faraday Discuss. (2020).

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• Saptarshi Chakraborty, Milka Doktorova, Trivikram R. Molugu, Frederick A. Heberle, Haden L. Scott, Boris Dzikovski, Michihiro Nagao, Laura-Roxana Stingaciu, Robert F. Standaert, Francisco N. Barrera, John Katsaras, George Khelashvili, Michael F. Brown, and Rana Ashkar. “How cholesterol stiffens unsaturated lipid membranes.” PNAS (2020). PMID: 202004807

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• Palanikumar L, Al-Hosani S, Kalmouni M, Nguyen VP, Ali L, Pasricha R, Barrera FN, Magzoub M. “pH-responsive high stability polymeric nanoparticles for targeted delivery of anticancer therapeutics.” Communications Biology (2020). PMID: 32127636

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• Svoronos AA, Bahal R, Pereira MC, Barrera FN, Deacon JC, Bosenberg M, DiMaio D, Glazer PM, Engelman DM. “Tumor-Targeted, Cytoplasmic Delivery of Large, Polar Molecules Using a pH-Low Insertion Peptide.” Mol Pharm (2020). PMID: 31855437

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• Bañó-Polo M, Martínez-Gil L, Barrera FN, Mingarro I. “Insertion of Bacteriorhodopsin Helix C Variants into Biological Membranes.” ACS Omega (2020). PMID: 31956802

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• Westerfield JM, Barrera FN. “Membrane receptor activation mechanisms and transmembrane peptide tools to elucidate them.” JBC (2020). PMID: 31879273

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2019

• Nguyen MHL, DiPasquale M, Rickeard BW, Doktorova M, Heberle FA, Scott HL, Barrera FN, Taylor G, Collier CP, Stanley CB, Katsaras J, Marquardt D. “Peptide-Induced Lipid Flip-Flop in Asymmetric Liposomes Measured by Small Angle Neutron Scattering.” Langmuir (2019). PMID: 31408345

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• Westerfield JM*, Gupta C*, Scott HL*, Ye Y, Cameron A, Mertz B, Barrera FN. “Ions Modulate Key Interactions between pHLIP and Lipid Membranes.” Biophysical Journal (2019). PMID: 31422821 *contributed equally

PDF | URL | DOI | Commentary by Jonathan Schlebach

• Nguyen VP, Dixson AC, Barrera FN. “The Effect of Phosphatidylserine on a pH-Responsive Peptide Is Defined by Its Noninserting End.” Biophysical Journal (2019). PMID: 31400916

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• Nguyen VP, Palanikumar L, Kennel SJ, Alves DS, Ye Y, Wall JS, Magzoub M, Barrera FN. “Mechanistic insights into the pH-dependent membrane peptide ATRAM.” Journal of Controlled Release (2019). PMID: 30763623

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• Scott HL, Heberle FA, Katsaras J, Barrera F.N. “Phosphatidylserine Asymmetry Promotes the Membrane Insertion of a Transmembrane Helix.” Biophysical Journal (2019). PMID: 30954213

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• Tanei J. Ricks, Chelsi D. Cassilly, Adam J. Carr, Daiane S. Alves, Shahrina Alam, Kathrin Tscherch, Timothy W. Yokley, Cameron E. Workman, Jennifer L. Morrell‐Falvey, Francisco N. Barrera, Todd B. Reynolds, Michael D. Best. “Labeling of Phosphatidylinositol Lipid Products in Cells through Metabolic Engineering by Using a Clickable myo‐Inositol Probe.” ChemBioChem (2019). PMID: 30098105

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2018

• Daiane S Alves, Justin M Westerfield, Xiaojun Shi, Vanessa P Nguyen, Katherine M Stefanski, Kristen R Booth, Soyeon Kim, Jennifer Morrell-Falvey, Bing-Cheng Wang, Steven M Abel, Adam W Smith, Francisco N Barrera “A novel pH-dependent membrane peptide that binds to EphA2 and inhibits cell migration.” eLife (2018). PMID: 30222105

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• Zhang X, Alves DS, Lou J, Hill SD, Barrera FN, and Best MD. “Boronic acid liposomes for cellular delivery and content release driven by carbohydrate binding.” Chem Comm (2018). PMID: 29809225

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• Usery RD, Enoki TA, Wickramasinghe SP, Nguyen VP, Ackerman DG, Greathouse DV, Koeppe RE, Barrera FN, and Feigenson GW. “Membrane Bending Moduli of Coexisting Liquid Phases Containing Transmembrane Peptide.” Biophysical Journal (2018). PMID: 29742408

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 2017

• Alexander G. Karabadzhak, Lisa M. Petti, Francisco N. Barrera, Anne P. B. Edwards, Andrés Moya-Rodríguez, Yury S. Polikanov, J. Alfredo Freites, Douglas J. Tobias, Donald M. Engelman, and Daniel DiMaio. “Two transmembrane dimers of the bovine papillomavirus E5 oncoprotein clamp the PDGF β receptor in an active dimeric conformation.” Proceedings of the National Academy of Sciences (2017). PMID: 28808001

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• Haden L. Scott, Justin M. Westerfield, and Francisco N. Barrera. “Determination of the Membrane Translocation pK of the pH-Low Insertion Peptide.” Biophysical Journal (2017). PMID: 28834723

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 2015

• Vanessa P. Nguyen, Daiane S. Alves, Haden L. Scott, Forrest L. Davis, and Francisco N. Barrera. “A Novel Soluble Peptide with pH-Responsive Membrane Insertion.” Biochemistry (2015). PMID: 26497400    *Editor’s Choice

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• Alam, Shahrina, Daiane Santana Alves, Stuart A. Whitehead, Andrew Michael Bayer, Christopher D. McNitt, Vladimir V. Popik, Francisco Nicolas Barrera, and Michael D. Best. “A Clickable and Photocleavable Lipid Analog for Cell Membrane Delivery and Release.” Bioconjugate chemistry (2015). PMID: 25927978

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• Scott, Haden L., Vanessa P. Nguyen, Daiane S. Alves, Forrest L. Davis, Kristen R. Booth, Jordan Bryner, and Francisco N. Barrera. “The Negative Charge of the Membrane Has Opposite Effects on the Membrane Entry and Exit of pH-Low Insertion Peptide.” Biochemistry 54, no. 9 (2015): 1709-1712. PMID:25692747

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• Cheng, Christopher, Raman Bahal, Imran Babar, Zachary Pincus, Francisco Barrera, Connie Liu, Alexander Svoronos, Demetrios Braddock, Peter Glazer, Donald Engelman, W. Saltzman, and Frank Slack. “MicroRNA Silencing for Cancer Therapy Targeted to the Tumour Microenvironment.” Nature 518.7537 (2014): 107-10. PMID:25409146

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• Deacon, John, Donald Engelman, and Francisco Barrera. “Targeting Acidity in Diseased Tissues; Mechanism and Applications of the Membrane-inserting Peptide, pHLIP.” Archives of Biochemistry and Biophysics 565 (2015): 40-48. PMID:25444855

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2014

• CohenEB, Jun SJ, Bears Z, Barrera FN, AlonsoM, Engelman DM, DiMaio D. Mapping the homodimer interface of an optimized, artificial, transmembrane protein activator of the human erythropoietin receptor. PLoS One2014. PMID: 24788775

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• Kohlway A, Pirakitikulr N, Barrera FN, Potapova O, Engelman DM, Pyle AM, Lindenbach BD. Hepatitis C virus RNA replication and virus particle assembly require specific dimerization of the NS4A protein transmembrane domain. J Virol.2014; 88: 625-42. PMID: 24173222

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2013

• Fendos J, Barrera FN Engelman DM. Aspartate Embedding Depth Affects pHLIP’s Insertion pKa. Biochemistry2013; 52, 4595-604. PMID: 23721379  Highlighted on the journal

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2012

• Barrera FN, Fendos J, Engelman DM. Membrane physical properties influence transmembrane helix formation. Proc Natl Acad Sci USA2012; 109: 14422-7. PMID: 22908237

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2011

• Barrera FN, Weerakkody D, Anderson M, Andreev OA, Reshetnyak YK, Engelman DM. Roles of carboxyl groups in the transmembrane insertion of peptides. J Mol Biol.2011;413:359-71. PMID: 21888917

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2010

• Cammett TJ, Jun SJ, Cohen EB, Barrera FN, Engelman DM, DiMaio D. Construction and genetic selection of small transmembrane proteins that activate the human erythropoietin receptor. Proc Natl Acad Sci USA. 2010;107:3447-52. PMID: 20142506

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• Triano I, Barrera FN, Renart ML, Molina ML, Fernandez-Ballester G, Poveda JA, Fernandez AM, Encinar JA, Ferrer-Montiel AV, Otzen D, Gonzalez-Ros JM. Occupancy of nonannular lipid binding sites on KcsA greatly increases the stability of the tetrameric protein. Biochemistry. 2010;49:5397-404. PMID: 20481584

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2009

• Talbert-Slagle K, Marlatt S, Barrera FN, Khurana E, Oates J, Gerstein M, Engelman DM, Dixon AM, DiMaio D. Artificial transmembrane oncoproteins smaller than the bovine papillomavirus E5 protein redefine sequence requirements for activation of the platelet-derived growth factor beta receptor. J Virol. 2009;83:9773-85. PMID: 19605488

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• Neira JL, Roman-Trufero M, Contreras LM, Prieto J, Singh G, Barrera FN, Renart ML, Vidal M. The transcriptional repressor RYBP is a natively unfolded protein which folds upon binding to DNA. Biochemistry. 2009;48:1348-60. PMID: 19170609

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2008

• Molina ML, Barrera FN*, Encinar JA, Renart ML, Fernandez AM, Poveda JA, Santoro J, Bruix M, Gavilanes F, Fernandez-Ballester G, Neira JL, Gonzalez-Ros JM. N-type inactivation of the potassium channel KcsA by the Shaker B “ball” peptide: mapping the inactivating peptide-binding epitope. J Biol Chem. 2008;283:18076-85. PMID:  18430729

*Co-first author.

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• Barrera FN, Renart ML, Poveda JA, de Kruijff B, Killian JA, Gonzalez-Ros JM. Protein self-assembly and lipid binding in the folding of the potassium channel KcsA. Biochemistry. 2008;47:2123-33. PMID: 18205389

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• Barrera FN*, del Alamo M, Mateu MG, Neira JL. Envelope lipids regulate the in vitro assembly of the HIV-capsid. Biophysical J.2008;94:L8-10. PMID: 17981892

      Corresponding author.

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2007

• Alcaraz LA, Del Alamo M, Barrera FN, Mateu MG, Neira JL. Flexibility in HIV-1 assembly subunits: solution structure of the monomeric C-terminal domain of the capsid protein. Biophys J. 2007;93:1264-76. PMID: 17526561

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• Czypionka A, de los Panos OR, Mateu MG, Barrera FN, Hurtado-Gomez E, Gomez J, Vidal M, Neira JL. The isolated C-terminal domain of Ring1B is a dimer made of stable, well-structured monomers. Biochemistry. 2007;46:12764-76. PMID: 17935356

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2006

• Renart ML, Barrera FN, Molina ML, Encinar JA, Poveda JA, Fernandez AM, Gomez J, Gonzalez-Ros JM. Effects of conducting and blocking ions on the structure and stability of the potassium channel KcsA. J Biol Chem. 2006;281:29905-15. PMID: 16815844

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• Molina ML, Barrera FN, Fernandez AM, Poveda JA, Renart ML, Encinar JA, Riquelme G, Gonzalez-Ros JM. Clustering and coupled gating modulate the activity in KcsA, a potassium channel model. J Biol Chem. 2006;281:18837-48. PMID: 16670090

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• Barrera FN, Hurtado-Gomez E, Lidon-Moya MC, Neira JL. Binding of the C-terminal domain of the HIV-1 capsid protein to lipid membranes: a biophysical characterization. Biochem J. 2006;394:345-53. PMID: 16259620

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2005

• Hernandez JA, Meier J, Barrera FN, de los Panos OR, Hurtado-Gomez E, Bes MT, Fillat MF, Peleato ML, Cavasotto CN, Neira JL. The conformational stability and thermodynamics of Fur A (ferric uptake regulator) from Anabaenasp. PCC 7119. Biophys J. 2005;89:4188-200. PMID: 16169981

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• Barrera FN, Renart ML, Molina ML, Poveda JA, Encinar JA, Fernandez AM, Neira JL, Gonzalez-Ros JM. Unfolding and refolding in vitroof a tetrameric, alpha-helical membrane protein: the prokaryotic potassium channel KcsA. Biochemistry. 2005;44:14344-52. PMID: 16245951

     Considered of “special interest” in: Current Opinion in Structural Biology 2006, 16:480–488

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• Lidon-Moya MC, Barrera FN, Bueno M, Perez-Jimenez R, Sancho J, Mateu MG, Neira JL. An extensive thermodynamic characterization of the dimerization domain of the HIV-1 capsid protein. Protein Sci. 2005;14:2387-404. PMID: 16131662

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• Encinar JA, Molina ML, Poveda JA, Barrera FN, Renart ML, Fernandez AM, Gonzalez-Ros JM. The influence of a membrane environment on the structure and stability of a prokaryotic potassium channel, KcsA. FEBS Lett. 2005;579:5199-204. PMID: 16150445

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• Hurtado-Gomez E, Barrera FN, Neira JL. Structure and conformational stability of the enzyme I ofStreptomyces coelicolor explored by FTIR and circular dichroism. Biophys Chem. 2005;115:229-33. PMID: 15752610

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2004

• Molina ML, Encinar JA, Barrera FN, Fernandez-Ballester G, Riquelme G, Gonzalez-Ros JM. Influence of C-terminal protein domains and protein-lipid interactions on tetramerization and stability of the potassium channel KcsA. Biochemistry. 2004;43:14924-31. PMID:  15554699

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• Garzon MT, Lidon-Moya MC, Barrera FN, Prieto A, Gomez J, Mateu MG, Neira JL. The dimerization domain of the HIV-1 capsid protein binds a capsid protein-derived peptide: a biophysical characterization. Protein Sci. 2004;13:1512-23. PMID: 15152086

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2003

• Barrera FN, Poveda JA, Gonzalez-Ros JM, Neira JL. Binding of the C-terminal SAM domain of human p73 to lipid membranes. J Biol Chem. 2003;21:46878-85 PMID: 12954612

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• Muro-Pastor MI, Barrera FN, Reyes JC, Florencio FJ, Neira JL. The inactivating factor of glutamine synthetase, IF7, is a “natively unfolded” protein. Protein Sci.2003;12:1443-54. PMID: 12824490

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2002

• Barrera FN, Garzon MT, Gomez J, Neira JL. Equilibrium unfolding of the C-terminal SAM domain of p73. Biochemistry. 2002;41:5743-53. PMID: 11980478

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                      Invited Reviews and Correspondence

• Barrera FN, Neira JL. Turbulent times in southern European science. Lancet. 2013; 380:1229-30

PMID: 23040858

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• Barrera FN, Alcaraz LA, Hurtado-Gomez E, Neira JL. Into the lipid realm: stability and thermodynamics of membrane proteins. Curr Protein Pept Sci. 2008;9:626-37. PMID : 19075751

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